HI guys, so, i downloaded and installed Blast on my notebook but i have NO IDEA on how to use it. I downloaded a custom dataset from an article and i would like to run like a proteome against this dataset and exclude the similar proteins, remaining only the ones that are not similar. Like, i would like to compare the proteome i have with this data set and get only the non-similar proteins.
How can I do that?
p.s: i have no idea about anything, so, please, detail everything