I would like to use Ion Torrent data published by other for analysis. However, the only information I have by reading the article is the library was constructed using Ion Total RNASeq Kit v2 and Ion PI™ Template OT2 200 Kit v3. RNA-Seq was performed using the Ion PI™ Sequencing 200 kit v3 on the Ion Proton™ System
- I'm planning to use cutadapt to trim the adapter sequence from the raw data but may I know how to do so when there is no information of the adapter sequence used?
- How to determine the data is paired end reads or single-end read?