How to insert records in a mysql database with click path?

I have several files in a directory:

CHIKV1-X-gb_AB455493.fasta
CHIKV1-X-gb_AB455494.fasta
CHIKV1-X-gb_AB455494.fasta
...

File contents:

>lcl|NC_004162.2_cds_NP_690588.1_1 [locus_tag=CHIKVgp1] [db_xref=GeneID:956309] [protein=nonstructural polyprotein] [protein_id=NP_690588.1] [location=77..7501] [gbkey=CDS]
------------------------------------------------------------
----------------atggatcctgtgtacgtggacatagacgctgacagcgccttttt
gaaggccctgcaacgtgcgtaccccatgtttgaggtggaacctaggcaggtcacaccgaa
tgaccatgctaatgctagagcgttctcgcatctagctataaaactaatagagcaggaaat
tgatcccgactcaaccatcctggatattggtagtgcgccagcaaggaggatgatgtcgga
------------------------------------------------------------
------------------------------------------------------------
---------
>gb:AB455493|Organism:Chikungunya virus|Strain Name:SL11131|Segment:null|Host:Human
atggctgcgtgagacacacgtagcctaccagtttcttactgctctactctgcaaagcaag
agattaataacccatcatggatcctgtgtacgtggacatagacgctgacagcgccttctt
gaaggccctgcaacgtgcgtaccccatgtttgaggtggaaccaaggcaggtcacaccgaa
tgaccatgctaatgctagagcgttctcgcatctagctataaaactaatagagcaggaaat
tgaccccgactcaaccatcctggatatcggcagtgcgccagcaaggaggatgatgtcgga

I have this code:

import pymysql as MySQLdb
    from Bio import SeqIO
    import click
    import re
    from collections import defaultdict
    from Bio import AlignIO
    import os


def conexao():
    try:
        conn = MySQLdb.connect(
            host="localhost", user="root", passwd="", db=""
        )
        print("conectado")
        return conn
    except MySQLdb.Error as e:
        print(f"Erro ao conectar no Servidor MySQL: {e}")


def desconectar(conn):
    if conn:
        conn.close()


@click.command()
@click.option(
    "--path", default=".", type=click.Path(exists=True), help="Path para ser listado."
)
def ls2(path):
    with os.scandir(path) as d:
        for e in d:
            if not e.name.startswith(".") and e.is_file():
                lista_line = e.name
                for i in lista_line:
                    seqaln = AlignIO.read(lista_line, "fasta")
                    for record in seqaln:
                        id_alnRef = seqaln[0].id
                        print(id_alnRef)
                        id_alnG = seqaln[1].id
                        id_alnGE = re.search(r".*|", id_alnG)
                        id_alnGE = re.sub("|", " ", id_alnGE[0])
                        seq1 = seqaln[0].seq
                        seq2 = seqaln[1].seq
                        alinhamento = (
                            ">"
                            + id_alnRef
                            + "n"
                            + seq1
                            + "n"
                            + ">"
                            + id_alnGE
                            + "n"
                            + seq2
                            + "n"
                        )
                    conn = conexao()

    try:
        cursor = conn.cursor()
        cursor.execute(
            f"INSERT INTO tblAlinhamentos(FK_genomaID,FK_RefCdsID, seqAln)"
            f"VALUES( '{id_alnGE}', '{id_alnRef}','{alinhamento}');"
        )

        conn.commit()
        print(
            f"INSERT INTO tblAlinhamentos(FK_genomaID, FK_RefCdsID, seqAln)"
            f"VALUES('{id_alnGE}', '{id_alnRef}', '{alinhamento}');"
        )
    except MySQLdb.Error as e:
        print(f"Erro ao conectar no Servidor MySQL: {e}")


ls2()
desconectar(conn)

expected exit

PK_a  FK_genomaID FK_RefCdsID seqAln
1     gb:H3455      lc|xxxx    atgtatgat
2     gb:H3456      lc|xxxx    atgtatgat
3     gb:H3456      lc|xxxx    atgtatgat

however I cannot insert all files in the directory ... Only one or two contents of the files are inserted. In the directory there are more than two files. What am I doing wrong?