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2 hours ago by


Hi everyone,

I have a matrix of expression(counts) derived from 2 RNA experiments. I have combined the two experiments . I would like to normalize the data in away that at the end I have the expressions at the same scale and can do Differentially expression analysis.

What I have tried so far is using edgeR normalization method and limma package to remove the batches which failed to put the data in same scale.

Now I would like to try normalization with respect to House-Keeping genes which is new to me. I don't know how I can find those HKGs from the expression data. Is there any R package to detect those genes or I need to find them manually? If So, How should I do that?

The next step after finding the list of HKGs is the Normalization using this genes' expression where I need help too.

Thank you in advance

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