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2 hours ago by


For a list of mutations in SPDI format, I would like to fetch the flanking sequences, in order to idenitfy near sites that can be targeted with Crispr-Cas

The main issue is that in the list of SPDI identifiers is based on multiple different genome alignments.

Here is an example:


I have tried the rentrez package, but couldn't find a way to only download a particular fraction of the sequences.
Would appreciate any suggestions on how to approach this, including R-based approaches or using galaxy.


2 hours ago


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