How to confirm, using Seurat, whether a variation in the cell numbers between scRNA-seq experiment groups is because of the different treatments?

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I have 3 fish groups differently treated. With scRNA-seq and data analysis using Seurat, I found out there a big variation in the cell numbers/treatment. Given that the batch effect was controlled, and this variation is demonstrating the same pattern across all the replicates, what sort of analysis in Seurat can support or refute that this finding is attributed to the different treatments?


Seurat


UMI


histogram


scRNA-seq

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