gravatar for salehm

4 hours ago by

Hi,

I received RNA seq data for 55 samples run by illumina sequencer Nextseq500. Each sample has 4 fastq files and each file is in a separate directory. So I have a total of 220 directories, each directory has only one fastq file. Now I need to concatenate each 4 files (belong to their respective sample) in a single fastq file. I used to use this command:

"for i in $(find ./ -type f -name ".fastq.gz" | while read F; do basename $F | rev | cut -c 22- | rev; done | sort | uniq)
do echo "Merging R1"
cat "$i"_L00_R1_001.fastq.gz > "$i"_ME_L001_R1_001.fastq.gz
done"

However, it needs that all files to be in one directory. My files are now in 220 directories. So I am wondering if there is a way to modify this command to look for files in different directories. Or if there is a command, I could use to move each file in the individual directories to a single directory.

Thank you for your help.



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