Hello, I have a question related to analyzing Sanger sequenced data. The reference gene is >7 kb long and it has 4 CDS (1900 bp). The gene has 8 allelic forms (1900 bp). My goal is to re-sequence the 4 exons from my material and compare the allelic diversity (if there is any) with the reference alleles.
I have Sanger sequenced all my materials and obtained 4 exons for each genotype.
My question is how can I start analyzing this data and compare it against the reference alleles? Which software should I use and what should be my approach?
Can someone please give me a headstart?
Thank you very much.