gravatar for carlos_marchi

2 hours ago by

Brazil, São Paulo


I have DNA methylation samples from two groups of patients (case, control) where I would like to know if the differential methylation is significant and it is hypermethylated or hypomethylated.

Check differential methylation is significant:

pvalue < 0.05 and Delta M?

Is it correct to calculate delta M like this:
Delta M = mean(case_levels) - mean(normal_levels)

What it Delta M threshold? Is |delta_m| >= 2?

How to know the region is hypermethylated or hypomethylated?

hypermethylated if |delta_m| >= 2 otherwise hypomethylated?

Thanks a lot!

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