Hi to all,

I'm new in analyzing RNA-seq data. I have to evaluate gene expression in different cells and tissues available on online datasets. To perform DEA I need replicates of course. How can I be sure that these below are technical replicates?

![enter image description here][1]

They derive from the same experiment, I mean same protocol, same day of library preparation, same day of sequencing and same sequencing technology but they are different runs. In your opinion are they replicates?

Thank you in advance for your time and suggestions!

[1]: i.ibb.co/mBNq9Sb/GGG.png



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