gravatar for kristina.mahan

3 hours ago by

I want to run rnaQUAST on my transcriptome assembly on a yellow green algae. rnaQUAST says it needs a transcripts fasta file, genome assembly fasta file, and a gene coordinates gtf file from Ensembl or Gencode. Do I just try to find the closest related organism to my organism or how do I go about this?

python rnaQUAST.py
--transcripts /PATH/TO/transcripts1.fasta /PATH/TO/ANOTHER/transcripts2.fasta [...]
--reference /PATH/TO/reference_genome.fasta --gtf /PATH/TO/gene_coordinates.gtf



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