How convert fasta into Iso-seq bam?

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We know raw Iso-seq subreads in bam format which just store sequences and can be used to perform ccs, lima and cluster.

But if data from NCBI SRA database, the data are in fasta/fastq format,and I don't know how to process these data
These fasta/fastq data have polyA and primer sequences.

I want to remove primer sequences and orient these data in the 5′-3′ direction just like Lima can do.

Thanks a lot!


Iso-seq


subreads

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