GWA QC needed for combining genotyping array data from different chip versions

0

Dear all,

I have received a set of genotyping data in PLINK (bed) format.

  • Of these, around 950 European samples are genotyped using GSAv1 illumina array and around 70 samples are genotyped using illumina GSAv2 array.
  • 96% of SNP IDs match between these two chips versions.

I fed the genotyping data (of the shared SNPs) to my analysis pipeline and created the PC plots:

  • PCs of anchored samples for assigning population ancestry:

2105-Chip-Ancestry

  • PCs of EUR population before GWA. Results appear to overlap between chips [images below]

2105-GWA-PCs

However when I run GWA against chip versions (with 10 PCs and age as covariates), I get some significant hits [Image below].

210504-Chip-GWA

I would be very grateful for any recommendations/suggestions on how to go about combine the two genotyping datasets?

Many thanks for your feedbacks and time beforehand,


Chip


GWAS


batcheffect


QC

• 77 views

updated 2 hours ago by

▴

600

written 16 hours ago by

▴

30



Source link