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1 hour ago by

Mexico

As you pointed, you are predicting genes, any prediction program has some error rate in the prediction, the only way to get the real one will be with validation. In the lab, you can validate your genes simply by RT-PCR, design some primers to validate if your sequences are connected or not, extract RNA, and do the RT-PCR.
Also, 176 bp is too short to be a promoter, sound like it is an intronic region between exons, look for splicing signals.

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JC10k



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