Some standard Illumina libraries, such as Nextera, require the use of dsDNA-specific fluorescent dye methods for accurate quantification. These methods typically measure dsDNA concentration in ng/µl. To convert from ng/µl to nM for cluster generation, follow the instructions below.
1. Determine the average size of the library by running it on an Agilent Technologies 2100 Bioanalyzer.
2. Use the following formula to convert from ng/μl to nM:
( Concentration in ng/μl ) _______________________________ X 10^6 = concentration in nM ( 660 g/mol x average library size in bp )
Excuse me, I don't understand why we multiply in 660 and why 10^6 specifically?
Thanks in advance