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3 hours ago by

Hello Everyone

I am having two biological replicates obtained from one cell line by RNA-sequencing. The alignment was done using STAR and counts were generated using FeautreCounts. I am trying to see whether a gene is being expressed or not in the given cell line? I am not doing any differential gene analysis. My question is how to remove low expressed gene? Should I remove the genes showing zero counts or filter the genes after the normalization? Is it ok to use filterByExpr() if I don't have to compare between treated and control ? I tried to go through the other post but could not come to conclusions. I am looking forward for your suggestions. Thank you so much

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