Hello, I am analysing WGS data of human(50X tumor tissue). This Blog filter variants after Mutect2 call by FilterMutectCalls
. But GetPileupSummaries
requires huge memory(Even 500G is not enough), so my analysing keep failing at this step.
So I want to ask some experience to filter Mutect2 result without GetPileupSummaries
and CalculateContamination
. Thanks.