fastp results, per base content


Should I be concerned with the per base ratio content despite having good quality scoring on every base position? Per base content

Quality score






If you are concerned about the bias observed in the first 9 bases, that is absolutely normal in Illumina sequencing when a mechanical method of breaking the DNA to get the shotgun fragments in used. It turn out that DNA is more fragile in some sequences, and this is why you see it

It does not affect your analysis

I see in the second graphic a high quality even at the end

If you don't see GC bias and the presence of adapters and/or a high level of duplicated or overrepresented sequences, I would say that sequencing went just fine and you do not require to filter. You did not mention whether DNA or RNA is used

before adding your answer.

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