Extracting transcripts from whole-transcriptome libraries

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Hi there,

I am having trouble finding a tool that will successfully extract specific transcripts from whole transcriptome libraries. The transcriptome libraries were assembled through Trinity, and the reference transcriptome was a de novo assembly from Trinity also.

My ultimate goal is to extract the transcripts of interest, and then compare SNP counts and calculate dN/dS ratios. Any help is appreciated. Thanks.


galaxy


vcf


rna-seq

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