Extract subject sequences from Blast result
I ran TBLASTN (query=protein, subject=nucleotide) and got the following result:
qseqid sseqid pident sstart send evalue MP_11765 contig_17 95.322 5460 5221 0
I noticed that sstart > ssend meaning this query is mapping on the reverse complement of the subject. In this case how can I pull out the DNA sequence from my subject nucleotide file that corresponds to this hit? Any ideas on a program that can do this?
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