Extract reads counts with featureCounts
I'm trying to extract reads counts with featureCounts.
I obtained the gtf file from UCSC genome browsers through the command:
I ran the following command to extract reads counts
featureCounts -a dm6.ensGene.gtf -o Male_RNAseq.tx sorted.files/*bam
However, I got error that says
Failed to open the annotation file dm6.ensGene.gtf, or its format is incorrect, or it contains no 'exon' features.
Does anyone know how I can fix this error? I greatly appreciate any help, thank you
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