Error in using USEARCH - Bad line 5 in FASTQ file 'sample_R1.fastq': expected '@'

0

Hello, everyone. I'm running the 32-bit USEARCH to do the paired-reads merging and yet I'm having a pizzle which I don't know how to resolve.
 When using the command

However, I got an error message saying "Bad line 5 in FASTQ file 'sample1_R1.fastq' : expected '@'.

I've checked the input fastq file, and I am sure it's contained 4 lines with the first line the label line starting with @. So, I didn't see what the line 5 means here. ..And how do I do the trouble shooting?
Thanks for your help!


input


usearch


fastq


fastqfile

• 54 views



Source link