error in R

0

Can you help me why these errors happen?

$design
  groupCin. groupMet. groupNotreat.
1         0         0             1
2         1         0             0
3         0         1             0
attr(,"assign")
[1] 1 1 1
attr(,"contrasts")
attr(,"contrasts")$group
[1] "contr.treatment"

> cont.matrix <- makeContrasts(CinVsMet = groupCin. - groupMet. ,levels=design)
*Error in eval(ej, envir = levelsenv) : object 'groupCin.' not found*
> cont.matrix
               Contrasts
Levels          CinVsMet
  groupCin.            1
  groupMet.           -1
  groupNotreat.        0

fit.cont <- contrasts.fit(fit, cont.matrix)
 fit.cont <- eBayes(fit.cont)
*Error: evaluation nested too deeply: infinite recursion / options(expressions=)?*
> options(expressions=20000)
> fit.cont <- eBayes(fit.cont)
*Error: node stack overflow*


RNASeq

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