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2 hours ago by

Hola soy nueva usando trimmomatic y linnux use la siguiente linea de comando :

 [email protected]:/mnt/c/Users/acer/Documents/doctorado/Genoma 4IGSN1/4IgSN1$ java -jar /usr/share/java/trimmomatic.jar PE -threads 8 -phred33 1_HIg5N1_S1_L001_R1_001.fastq 2_HIg5N1_S1_L001_R2_001.fastq 1_HIg5N1_S1_L001_R1_001_paired.fastq 1_HIg5N1_S1_L001_R1_001_unpaired.fastq 2_HIg5N1_S1_L001_R2_001_paired.fastq 2_HIg5N1_S1_L001_R2_001_unpaired.fastq ILLUMINACLIP:../trimmomatichome/adapters/TruSeq3-PE.fa:2:40:15 LEADING:30 TRAILING:30 SLIDINGWINDOW:4:27 MINLEN:75

pero tengo estos 2 errores y no se como solucionarlos:

TrimmomaticPE: Started with arguments:                                                                                                                                   -threads 8 -phred33 1_HIg5N1_S1_L001_R1_001.fastq 2_HIg5N1_S1_L001_R2_001.fastq 1_HIg5N1_S1_L001_R1_001_paired.fastq 1_HIg5N1_S1_L001_R1_001_unpaired.fastq 2_HIg5N1_S1_L001_R2_001_paired.fastq 2_HIg5N1_S1_L001_R2_001_unpaired.fastq ILLUMINACLIP:../trimmomatichome/adapters/TruSeq3-PE.fa:2:40:15 LEADING:30 TRAILING:30 SLIDINGWINDOW:4:27 MINLEN:75       
E: Could not find '../trimmomatichome/adapters/TruSeq3-PE.fa'.



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