HISAT error: Encountered internal HISAT2 exception (#1)

1

Hello, I am using HISAT2 for aligning transcripts to the reference genome (rice).

I have build the index and the files are as follows:

irgsp_apr.dna.6.ht2
irgsp_apr.dna.5.ht2
irgsp_apr.dna.2.ht2
irgsp_apr.dna.1.ht2
irgsp_apr.dna.8.ht2
irgsp_apr.dna.7.ht2
irgsp_apr.dna.4.ht2
irgsp_apr.dna.3.ht2
irgsp_apr.dna.fa

I am running the following commands:
./hisat2 -x "/home/user/Desktop/hisat_index/irgsp_apr.dna" -p 4 --min-intronlen 10 --max-intronlen 10000 "/home/user/Desktop/cutadapt_DRR006571.fastq"

However, I am getting the following error:
Encountered internal HISAT2 exception (#1)
(ERR): hisat2-align exited with value 1

Kindly help


RNA-Seq

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