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2 hours ago by

Pakistan/Islamabad/COMSATS

Hi everyone,
I am trying to perform DEG analysis. I've two samples each has 3 replicates (one is XR and other is YR) replicates are XR1,XR2,XR3 and YR1,YR2,YR3..

d<- DGEList(counts=as.matrix(raw.data[,2:7]), lib.size=library.sizes, group=expt)
d<- calcNormFactors(d)

At the following step, I got a warning message

d<- estimateCommonDisp(d)
Warning message:
In estimateCommonDisp.DGEList(d) :
  There is no replication, setting dispersion to NA.
d$common.dispersion
[1] NA

How to resolve this?

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modified 48 minutes ago

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2 hours ago
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Dr.Animo10



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