I am trying to perform DEG analysis. I've two samples each has 3 replicates (one is XR and other is YR) replicates are XR1,XR2,XR3 and YR1,YR2,YR3..
d<- DGEList(counts=as.matrix(raw.data[,2:7]), lib.size=library.sizes, group=expt) d<- calcNormFactors(d)
At the following step, I got a warning message
d<- estimateCommonDisp(d) Warning message: In estimateCommonDisp.DGEList(d) : There is no replication, setting dispersion to NA. d$common.dispersion  NA
How to resolve this?