gravatar for ATpoint

41 minutes ago by

Germany

DESeq2, as clearly states in the manual, starts from raw counts.

If you want the normalized counts either use the code you show or, if you need it for clustering or similar applications, better transform the raw data with vst to get variance-stabilized counts on approximately log2-scale. Please read the manual, this is all in the FAQ section.

bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html



Source link