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2 hours ago by

Hi I'm a novice for ChIP-seq and trying to use DiffBind.

I have ChIP-seq dat of WT and mutant1 for H3K27me3. Each genotype has 2 replicates.

When I try the differential analysis, I get an error message "2 groups and at least triplicates for each group is required".

I understand triplicates are kinda minimum with meaningful stats but as a preview I wonder if I can run with duplicates before I generate more replicates.

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jc28420



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