Detection of heterozygous exon deletion by Sanger and Long-Range PCR

1

Hello,
May I ask is it possible to detect a heterozygous exon deletion (50 bps) by Sanger sequencing using the quality scores of the region? Also can it be detected by Long-Range PCR, please?
What is the best method to detect this deletion, please?
Thank you


Sanger


sequencing


PCR


Long-Range

• 89 views

I wouldn't try to call such things unless I could eyeball the traces, and confirm the boundaries of the indel. I would not rely only on per base quality scores.


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