Detection of bacterial SNP using regular PCR primers

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Hi all,

I have two bacterial strains that only differ from several SNPs in their genomes.
I am wondering if designing specific primer sequences containing the SNP mutation will be enough to differentiate the two strains using regular PCR?

I have heard of locked nucleic acid oligonucleotides but I would rather use regular PCR if possible.

Thank you in advance for you much appreciated help and advices!

Audrey


detection


PCR


SNP

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