gravatar for Kevin Blighe

1 hour ago by

Americas / Europe

It sounds like you want to do a likelihood ratio test in order to capture genes whose variances differ across your four groups. If this is the case, then you will likely want to perform, for example:

dds <- DESeqDataSetFromMatrix(..., design= ~ group)
dds <- DESeq(dds, test = 'LRT', reduced = ~1)
res <- results(dds)

Please take a look at the 'likelihood ratio test' section of the vignette.

Kevin



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