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5 hours ago by

I would like to perform differential expression analysis on a scRNA-sequenced GEO dataset (GSE111065). I can use either raw counts from featureCounts, or normalized counts prepared via the deconvolution method in scran. I would like to use the normalized counts if possible, but am unsure if I can use them as input into programs like Seurat. I'm interested in figuring our how a few very specific genes change between cells of different types.



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