Cuffdiff for DEGs

0

Hi, I am using cuffdiff for the Differential Gene Expression. Upon running the command, I see this problem. I have only three samples there is no replicate but my data is running accurately. I am confused, either it will cause a problem in the future or not?

Outputs:

No conditions are replicated, switching to 'blind' dispersion method
Inspecting maps and determining fragment length distributions.
Modeling fragment count overdispersion.


DEGs


Cuffdiff

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