Creating reference genome for mapping and then selecting

0

Dear community,

I will be analyzing PDx RNA-seq data and whatever information I could gather is that I need couple of things before I start:-

  1. Combined reference genome of mouse and human (hg38 and mm10).

How can I generate this from hg38.fa and mm10.fa files?

  1. Using combined reference genome for alignment using STAR.

What special features do I need to use so that only the reads that exclusively map to hg38 are selected and a Gene count can be generated.

Could anyone of you help me.

Thank you in advance


mm10


hg38


STAR


RNA-seq

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