Creating Annotation GTF/GFF file

1

Hello everyone, I have human Rna-Seq data reading by MiniOn.I aligned with the human reference genome (GRCh38.p13) from that website= www.gencodegenes.org/human/ using minimap2. Then I extract specific regions on the bam file using samtools. For the starting DESeq2, I tried to featureCounts with reference annotation folder bam file %5 assigned according to multiqc report.

How to create an annotation folder for quantification?


HG38


Annotation


Rna-Seq


Genome

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