I am attempting to use the af-dist bcftools plugin to detect potential strand issues using the instructions found here: samtools.github.io/bcftools/howtos/plugin.af-dist.html
bcftools annotate -c INFO/AF -a af.vcf.gz DAC14_chr1_hg38_nonduplicates_fix.vcf.gz | bcftools +af-dist | grep ^PROB > DAC14_chr1_hg38_nonduplicates_fix.dist.txt
Failed to open DAC14_chr1_hg38_nonduplicates_fix.vcf.gz: could not load index
I checked to confirm that my data is there and it is the correct vcf.gz filetype needed:
fileformat=VCFv4.2 FILTER=<ID=PASS,Description="All filters passed"> fileDate=20210211
What's going wrong here? Tried to search, but no luck... Thank you.