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2 hours ago by

Hi, I'm new to RNA-seq and was assigned this differential expression project. I used edgeR to calculate my counts to CPM, but my advisor wants me to convert this to FPKM.

I'm also reading that it's probably preferred to use TMM for normalization, but I'm not entirely sure what the differences are, if there's upsides/downsides to one or the other, or if this is the proper thing to do (like is CPM sufficient for differential expression analysis)?

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igor9.2k

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2 hours ago
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klorilla0



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