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2 hours ago by

If you were interested in only 5 transcripts why did you go for RNA-seq. A simple real-time PCR would have been a lot cheaper.
Moreover, DE tools borrow information across genes for modelling the counts and estimation of parameters so I don't think its a good idea to just focus on 5 of them for downstream analysis. If you have some other independent ways to verify those 5 transcripts then it may be acceptable.

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ashish290



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