Please I need your input on how best to go about an rnaseq analysis I am currently working on as I couldnt find any closely related post. I have 5 datasets (4 with UMI counts and 1 with FPKM) to compare. I am taking the z-score of all the dataset separately before passing on to Seurat..
My questions are :
- Is this a right direction or there is a better way around?
-If it is the right approach, is there a need to do any normalization/log transformation/what normalization approach would be the best before merging or how best can one preprocess the datasets to be able get any valuable insight from the analysis?
- Is it possible to convert UMI to FPKM and then follow the Seurat Multiple Dataset Integration guide to go by the comparison?