I am new to R and scRNA seq analysis. I followed Bioconductor's tutorial to analyze a scRNA seq dataset, and now I am stuck with cell annotation.
Lots of people recommend the celldex and SingleR packages for annotation, but I am working on a kidney dataset and the annotated datasets in celldex did not help me. I applied BlueprintEncodeData() and HumanPrimaryCellAtlasData() on my dataset and found cell types like "astrocytes" and "gametocytes" which do not exist in kidney.
I wonder how can I annotate my cell clusters. Any response would be appreciated.