gravatar for rogerbear

2 hours ago by

Hi guys,

I am new to R and scRNA seq analysis. I followed Bioconductor's tutorial to analyze a scRNA seq dataset, and now I am stuck with cell annotation.

Lots of people recommend the celldex and SingleR packages for annotation, but I am working on a kidney dataset and the annotated datasets in celldex did not help me. I applied BlueprintEncodeData() and HumanPrimaryCellAtlasData() on my dataset and found cell types like "astrocytes" and "gametocytes" which do not exist in kidney.

I wonder how can I annotate my cell clusters. Any response would be appreciated.


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