Can not detect spliced / unspliced counts



I keep running into an error whenever trying to run an scVelo analysis, once my data is loaded (tried different formats, like mtx and csv), I get the error

WARNING: Could not find spliced / unspliced counts.

I have generated these files directly from the barcodes.tsv, genes.tsv, and matrix.mtx provided in the filtered output of the cellranger pipeline. Also tried it with the unfiltered ones and still getting the same error.

Would anyone be able to let me know if I'm missing something basic, or should I try to get these from somewhere else?
CellRanger pipeline v 3.1.0 was run on transcriptome GRCh38-3.0.0

Thanks you for any info.


RNA velocity



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