gravatar for jimmy0958073736

2 hours ago by


Hi everyone
I met some confusing issues in edgeR. and DEG
Right now I have three samples without any replicated.
I wonder to know to have any possibility to combine three samples for analysis (With the replicated sample, I used DEseq2 than before but right now I don't have any idea to analyze)

data <- read.csv("Vero.D2.normalisedReadCounts.csv")
bcv <- 0.2
 counts <- data
    y <- DGEList (counts=counts, group=1:2)
    et <- exactTest(y, dispersion=bcv^2)

If I can adjust upon code to achieve my goal.

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