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5 hours ago by

I have 151 fasta assemblies (contigs) corresponding to the fungal genome with a size of 50 Mb, including the reference sequence and I would like to perform a pangenome in order to have a single fasta sequence combining the 151 strains.

At first I mapped each of these assemblies to reference sequence then I extracted the bam file that contained unmapped reads from each stub, then I am stuck for the rest of the steps.

How is it possible to perform a single reference sequence based on the pangenome of these sequences?

Thank you,

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