CMD:

 /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn -query /home/sun/data/softwares/evaluation/TransDecoder-TransDecoder-v5.5.0/SRR3632057.trim_trinity.cdhit.fasta.transdecoder_dir/longest_or
    fs.cds  -db nucldb/nucleotideDB -out nucl_blastn_1align_1e-30_1.txt -num_alignments 1 -evalue 1e-30 -num_threads 10 > blastn_2.log

Total no. of fasta sequences - 149532, I tried to run with the less sequences, pops up the same error. I checked in the forum, their was similar issues posted in the forum wasn't so helpful, currently I'm using ncbi-blast-2.11.0+. Suggestions, please.

**Error: NCBI C++ Exception:**
    T0 "/home/coremake/release_build/build/PrepareRelease_Linux64-Centos_JSID_01_400117_130.14.18.128_9008__PrepareRelease_Linux64-Centos_1601968763/c++/compilers/unix/../../src/corelib/ncbiobj.cpp", line 981: Critical: (CCoreException::eNullPtr) ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL pointer.
    T0 "/home/coremake/release_build/build/PrepareRelease_Linux64-Centos_JSID_01_400117_130.14.18.128_9008__PrepareRelease_Linux64-Centos_1601968763/c++/compilers/unix/../../src/app/blast/blast_app_util.cpp", line 789: Critical: (CCoreException::eNullPtr) BLAST::ncbi::BlastFormatter_PreFetchSequenceData() - Error pre-fetching sequence data 
     Stack trace:
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 ncbi::CObject::ThrowNullPointerException() offset=0x2D5 addr=0x1c667d5
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 ncbi::objects::CBioseq_Handle::x_GetInfo() const offset=0x1A addr=0x159167a
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 ncbi::objects::CBioseq_Handle::IsSetInst_Length() const offset=0x6 addr=0x15937b6
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 ncbi::objects::CBioseq_Handle::GetBioseqLength() const offset=0xB addr=0x159371b
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 ncbi::blast::LoadSequencesToScope(std::vector<ncbi::objects::CSeq_id_Handle, std::allocator<ncbi::objects::CSeq_id_Handle> >&, std::vector<ncbi::CRange<unsigned int>, std::allocator<ncbi::CRange<unsigned int> > >&, ncbi::CRef<ncbi::objects::CScope, ncbi::CObjectCounterLocker>&) offset=0x3AC addr=0xed386c
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 ncbi::BlastFormatter_PreFetchSequenceData(ncbi::blast::CSearchResultSet const&, ncbi::CRef<ncbi::objects::CScope, ncbi::CObjectCounterLocker>, ncbi::blast::CFormattingArgs::EOutputFormat) offset=0x7F addr=0xa5522f
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 CBlastnApp::Run() offset=0xFD0 addr=0xa4ba70
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 ncbi::CNcbiApplicationAPI::x_TryMain(ncbi::EAppDiagStream, char const*, int*, bool*) offset=0x833 addr=0x1b184a3
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 ncbi::CNcbiApplicationAPI::AppMain(int, char const* const*, char const* const*, ncbi::EAppDiagStream, char const*, std::__cxx11::basic_string<char, std::char_traits<char>, std::allocator<char> > const&) offset=0x93B addr=0x1b16f6b
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 main offset=0x88 addr=0xa4a938
      /usr/lib64/libc.so.6 ???:0 __libc_start_main offset=0xF5 addr=0x7f43b96da505
      /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn ???:0 /home/sun/data/softwares/ncbi/ncbi-blast-2.11.0+/bin/blastn() [0xa4a7f5] offset=0x0 addr=0xa4a7f5



Source link