Hi, I'm now considering a confusing question for next-generation sequencing.
Suppose I have a cell with 4 genes, and with a consistent expression quantity of 1:1:1:1. In wild type, their expressions are 50:50:50:50 and in mutant are 500:500:500:500. But because of the sequencing method, samples need to be extracted and PCR, then select some for sequencing mechine. And finally I get some DNA to sequence. Here comes a question, if I get 1G data from wild and mutant sample, but they may show the sample expression quantity of 5:5:5:5. How can I cope with the problem like this or in the realitistic data? Thank you!