ATAC-seq protocol stop-point check
Hi everyone! I'm performing ATAC-seq to determine open chromatin regions of a cell line. I went through the protocol and wonder if there are means to verify during the whole procedure if we are getting a result or even a glimpse of the result? Like a stop-point and see a preview of the end-product. Is there such a way? or the closest thing is performing library-quality through bioanalyzer after PCR amplification? Thank you very much in advance.
Oh, there is actually a stopping point before PCR amplification, wherein we can store the purified DNA at -20 after tagmentation and before PCR amplification. Is there a way or possibility that at this stop point, we can have a preview of the result or check whether we are doing the right thing?
Thanks once again. Keep safe!
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