Argument "query". Incompatible with argument: `in_pssm' blast+

1

hello, i have a commande in old blast
**

blastall -i $refseq -d $seqFilename -p psitblastn -R $chkpFile -F F -M BLOSUM62 -t -1 -e $max_Evalue -v 10000 -b 10000 > $outputFile

**
and i want to stranlsted to new blast+, i did as fellow i'm not really sure if i got it right

tblastn -query $refseq -db $seqFilename -in_pssm $chkpFile  -seg no -matrix BLOSUM62 -max_intron_length 1 -evalue $max_Evalue -num_descriptions 10000 -num_alignments 10000 -out $outputFile

i have changed mac intron value from the olad blast which is -1 to 1 in blast+
and i got the fellowing error wich is normale because in tblastn -help says

-query <File_In>    Input file name    Default = `-'
    * Incompatible with:  in_pssm

how can i translate the commande above


blast


ncbi


psiblast


tblastn

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