Are there risks to use a GRCh38 gtf and a hg19 fa in alignment?

1

We have used STAR program for aligning RNA sequences against hg19 genome but I noticed the programmer has used a hg19 fasta file and a Gencode v30 (for GRCh38) gtf for annotation and counts. Is there any risks behind this alignment?


RNASeq

• 62 views



Source link