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4 hours ago by

Hello, Guys!

I am doing a research about Dendrobium officinale (a plant specie) RNA-seq.

I downloaded this specie Genome files (.fna and .gtf, enter link description here) to construct the "index file" (By RSEM-prepare-reference).

And I was ready to analyze some datasets (enter link description here) for my resarch.

However, I used RSEM to cacaulte the expression rate of RNA-seq datasets but the alignment rates were very low for RNA-seq datasets:

   20189357 reads; of these:
  20189357 (100.00%) were paired; of these:
    20163475 (99.87%) aligned concordantly 0 times
    6047 (0.03%) aligned concordantly exactly 1 time
    19835 (0.10%) aligned concordantly >1 times
0.13% overall alignment rate

I don't know why. Could anyone solve for me?! Thanks too much!

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