3' DGE (3′-digital gene expression) for knockdown analysis

0

Hi all,

I wonder if anyone used the 3'DGE (3′-digital gene expression) approach to measure the knockdown of target genes. I ask this because I have read that 3'end is not a good measure of knockdown. As you can see from this figure 3'end is definitely not preferred for measuring knockdown of target genes

enter image description here

Please let me know your experience using 3' DGE for knockdown or as a general approach for performing differentially gene expression and other downstream analysis and finally arriving at biological conclusions.

Thanks,

Upendra


DGE


3


knockdown


prime

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